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M9480830.TXT
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1994-09-05
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Document 0830
DOCN M9480830
TI Genetic and serological analysis of Pneumocystis carinii genes encoding
major surface glycoprotein (MSG).
DT 9410
AU Sunkin SM; Stringer SL; Stringer JR; University of Cincinnati College of
Medicine, Ohio.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:597 (abstract no. F-52). Unique
Identifier : AIDSLINE ASM94/94313115
AB Pneumocystis carinii contains a large (90-120 kDa) surface glycoprotein
that is recognized by antibodies in the serum of immune and infected
hosts and seems to play a role in binding to host cells. Recent genetic
analysis of MSG cDNAs has shown that MSG is likely to be a family of
proteins. A fragment from the genome of rat-derived P. carinii was found
to contain two MSG genes arranged as a direct repeat. The sequences from
one gene (MSG B), the region between the two genes, and part of the
second gene (MSG A) were determined. The open reading frames of MSG A
and MSG B encode non-identical proteins, both of which are similar to
that encoded by a previously published cDNA. The 5' and 3' untranslated
regions of the MSG gene pair were highly conserved. Genetic analysis of
MSG genes and cDNAs has assisted in defining presumptive variable and
constant regions of the MSG proteins, which can then be utilized in
examining the expression of this protein family. In another approach to
define variable and constant regions, an expression library of
rat-derived P. carinii was screened with monoclonal antibodies that
recognize MSG from rat-derived and human-derived P. carinii. Some of
these monoclonal antibodies appear to recognize an epitope that varies
among MSG proteins, while two others may recognize epitopes that are
conserved among these proteins.
DE Animal Antibodies, Monoclonal Antigenic Determinants/ANALYSIS
Comparative Study Fungal Proteins/ANALYSIS/BIOSYNTHESIS/*GENETICS Gene
Library *Genes, Fungal Human Membrane
Glycoproteins/ANALYSIS/BIOSYNTHESIS/*GENETICS Open Reading Frames
Pneumocystis carinii/*GENETICS/ISOLATION & PURIF/METABOLISM Rats
Repetitive Sequences, Nucleic Acid Species Specificity MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).